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Ultraviolet-Visible Spectrophotometric method

- Jul 19, 2017 -

Ultraviolet-visible spectrophotometric method is used to determine the absorbance of the substance in the 190~800nm wavelength range for identification, impurity inspection and quantitative determination. When light passes through the measured material solution, the absorption of the matter varies with the wavelength of light. Thus, by measuring the absorbance at different wavelengths and drawing the relationship between absorbance and wavelength, the absorption spectra of the substance are measured. The maximum absorption wavelength λmax and the minimum absorption wavelength λmin can be determined from absorption spectra. The absorption spectrum of the material has the characteristic of its structure. Therefore, the material can be identified by comparing the spectra of samples within a specific wavelength range with the spectra of control or control, or by determining the maximum absorption wavelength, or by measuring the absorption ratios at two specific wavelengths. The absorbance of a certain concentration sample solution is measured at the maximum absorption wavelength, compared with the absorbance of a certain concentration of control solution, or the concentration of the sample solution is calculated by absorption coefficient method.

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