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Ultraviolet-visible spectrophotometric method of wavelength

- Jul 19, 2017 -

Because of the influence of the environmental factors on the mechanical part, the wavelength of the instrument is often slightly changed, so the measuring wavelength should be measured before the calibration of the instruments used. The strong spectral lines in mercury lamps are $number 83nm, $number. 65nm, $number. 28nm, $number. 73nm, $number. 16nm, $number. 15nm, $number. 02nm, $number. 66nm, $number. 07nm and $number. 96nm; The $number of the deuterium lamp in the instrument. 02nm and 656.10nm line are corrected; Holmium glass at Wavelength $number. 4nm, $number. 5nm, $number. 7nm, $number. 9nm, $number. 5nm, 536.2 NM and 637.5nm have sharp absorption peaks, can also be used for wavelength correction, but because of different sources or over time will have a small change, use should be noted; In recent years, the use of perchloric acid and holmium solution of two-beam equipment, using 10% perchloric acid solution as a solvent, the preparation of the solution containing Holmium (Ho203) 4%, The absorption peak wavelength of the solution is $number. 13nm, $number. 10nm, $number. 18nm, $number. 44nm, $number. 47nm, $number. 31nm, $number. 28nm, $number. 30nm and $number. 29nm.

The allowable error of the instrument wavelength is: ultraviolet light area ±1nm, 500nm near ±2nm.


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