Single Beam UV VIS Spectrophotometer
The analytical sample and the standard sample were prepared in the same solvent at the same concentration, and the ultraviolet visible absorption spectrum was measured under the same conditions. If the two are the same substance, the spectra of the two should be identical. If there is no standard, it can also be compared with the existing standard spectrum. This method requires accurate, high precision and the same measurement conditions.
Experiments have shown that different polar solvents produce different hydrogen bond strengths, which can be determined by UV spectroscopy to determine the hydrogen bond strength of the compound in different solvents to determine which solvent to choose.