ultraviolet-visible absorption spectrum Principle
The ultraviolet-visible absorption spectrum of a molecule is an absorption spectrum generated by the transition of an electron level after absorption of ultraviolet visible radiation by certain groups in the molecule. Since various substances have different molecules, atoms and different molecular spatial structures, their absorption of light energy will not be the same.
Therefore, each substance has its own unique and fixed absorption spectrum curve, which can be absorbed according to absorption. The determination of the absorbance at certain characteristic wavelengths on the spectrum or the determination of the content of the substance is the basis for qualitative and quantitative analysis of spectrophotometry.